Bài đăng

Hiển thị các bài đăng có nhãn Multiplex PCR

Evaluation of the optimal multiplex PCR method for the detection of aspergillus flavus and aspergillus parasiticus on dried peanut

Nguyen, Thi Hue Aspergillus flavus and Aspergillus parasiticus areq the common aflatoxin producing species that usually infect onq foodstuff in their production line from the field to the storageq place such as peanut, corn, cereal, etc., especially in tropicalq country like Vietnam. Aflatoxins which are considered as derivedq secondary metabolites assigned as a group of mycotoxinsq produced by several species of the Aspergillus spp are potentq hepatotoxins, immunosuppression, carcinogen that lead toq mortality or reducing the productivity of farm animals. Thereq has been a demand for effective method to detect these twoq species on dried food. In previous study, a multiplex PCR methodq were designed to improve the detection process of A. flavusq and A. parasiticus and that method showed high sensitivityq and specificity by being applied on artificially infected driedq peanut. In this study, that multiplex PCR method would beq evaluated by testing the presence of A. flav...