Evaluation of the optimal multiplex PCR method for the detection of aspergillus flavus and aspergillus parasiticus on dried peanut
Nguyen, Thi Hue
Aspergillus flavus and Aspergillus parasiticus areq the common aflatoxin
producing species that usually infect onq foodstuff in their production
line from the field to the storageq place such as peanut, corn, cereal,
etc., especially in tropicalq country like Vietnam. Aflatoxins which
are considered as derivedq secondary metabolites assigned as a group of
mycotoxinsq produced by several species of the Aspergillus spp are
potentq hepatotoxins, immunosuppression, carcinogen that lead toq
mortality or reducing the productivity of farm animals. Thereq has been a
demand for effective method to detect these twoq species on dried food.
In previous study, a multiplex PCR methodq were designed to improve the
detection process of A. flavusq and A. parasiticus and that method
showed high sensitivityq and specificity by being applied on
artificially infected driedq peanut. In this study, that multiplex PCR
method would beq evaluated by testing the presence of A. flavus and A.
parasiticusq on natural dried peanut kernels. On this purpose, the
presenceq of A. flavus and A. parasiticus on the collected peanut from
theq market was determined using two method, the conventionalq culturing
method in Institute of Hygiene and Public Healthq (IHPH) and the
mentioned multiplex PCR. The efficiency ofq multiplex PCR method would
be evaluated by comparing fungiq detection result of two methods using
appropriate statisticalq tests. Next, fungal enrichment with distilled
water overnightq was applied to increase the detection percentage if the
firstq analysis do not get the expected result. The result showed thatq
54% results from PCR method was the same with culturingq method, and
after fungal enrichment, this percentage increasedq to 76% which
suggested that these two method was notq significantly different with
each other. Therefore, this multiplexq PCR method could have more
advanced points than theq culturing method in detection of A. flavus and
A. parasiticus onq foodstuff.
Chi tiết xin mời tham khảo tại http://repository.vnu.edu.vn/handle/VNU_123/34198
Title: | Evaluation of the optimal multiplex PCR method for the detection of aspergillus flavus and aspergillus parasiticus on dried peanut |
Authors: | Nguyen, Thi Hue |
Keywords: | Dried peanut kernels;Multiplex PCR;Culturing method;Aspergillus parasiticus;;Aspergillus flavus;Biomedical engineering |
Issue Date: | 2015 |
Publisher: | Springer Verlag |
Citation: | Scopus |
Description: | IFMBE Proceedings, Volume 46, 2015, Pages 367-370, 5th International Conference on the Development of Biomedical Engineering, 2014; Ho Chi Minh City; Viet Nam; 16 June 2014 through 18 June 2014; Code 117119 |
URI: | http://link.springer.com/chapter/10.1007%2F978-3-319-11776-8_90 http://repository.vnu.edu.vn/handle/VNU_123/34198 |
ISBN: | 978-331911775-1 |
Appears in Collections: | Bài báo của ĐHQGHN trong Scopus |
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